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bioRxiv · 10.1101/558098

Single mRNP analysis by super-resolution microscopy and fluorescence correlation spectroscopy reveals that small mRNP granules represent mRNA singletons

Mateu-Regue, A.·Christiansen, J.·Bagger, F. O.·Hellriegel, C.·Nielsen, F. C.
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Abstract

Small cytoplasmic mRNP granules are implicated in mRNA transport, translational control and decay. Employing Super-resolution Microscopy and Fluorescence Correlation Spectroscopy, we analyzed the molecular composition and dynamics of single cytoplasmic YBX1_IMP1 mRNP granules in live cells. Granules appeared elongated and branched with patches of IMP1 and YBX1 distributed along mRNA, reflecting the attachment of the two RNA-binding proteins in cis. Particles form at the nuclear pore and are spatially segregated from translating ribosomes, so the mRNP is a repository for mRNAs awaiting translation. Individual mRNPs contain a single mRNA and 5 to 15 molecules of YBX1 and IMP1, which is in agreement with the average number of mRNA-binding sites calculated from CLIP analyses. We conclude that small cytoplasmic mRNP granules are mRNA singletons, thus depicting the cellular transcriptome. Consequently, expression of functionally related mRNAs in RNA regulons is unlikely to result from coordinated assembly. O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY Graphical abstract C_FIG_DISPLAY

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Mateu-Regue, A., Christiansen, J., Bagger, F. O., Hellriegel, C., Nielsen, F. C.. 2019-02-22. Single mRNP analysis by super-resolution microscopy and fluorescence correlation spectroscopy reveals that small mRNP granules represent mRNA singletons. https://doi.org/10.1101/558098

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