Mixed-polarity random-dot stereograms alter GABA and Glx concentration in the early visual cortex

Abstract

The offset between images projected onto the left and right retinae (binocular disparity) provides a powerful cue to the three-dimensional structure of the environment. It was previously shown that depth judgements are better when images comprise both light and dark features, rather than only dark or only light elements. Since Harris and Parker (1995) discovered the "mixed-polarity benefit", there has been limited evidence supporting their hypothesis that the benefit is due to separate bright and dark channels. Goncalves and Welchman (2017) observed that single- and mixed-polarity stereograms evoke different levels of positive and negative activity in a deep neural network trained on natural images to make depth judgements, which also showed the mixed-polarity benefit. Motivated by this discovery, here we seek to test the potential for changes in the balance of excitation and inhibition that are produced by viewing these stimuli. In particular, we use magnetic resonance spectroscopy to measure Glx and GABA concentration in the early visual cortex of adult humans while viewing single- and mixed-polarity random-dot stereograms (RDS). We find that observers Glx concentration is significantly higher while GABA concentration is significantly lower when viewing mixed-polarity RDS than when viewing single-polarity RDS. These results indicate that excitation and inhibition facilitate processing of single- and mixed-polarity stereograms in the early visual cortex to different extents, consistent with recent theoretical work (Goncalves & Welchman, 2017).