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bioRxiv · 10.1101/525345

Stoichiometric quantification of spatially dense assemblies with qPAINT

Baker, M.·Nieves, D. J.·Hilzenrat, G.·Berengut, J. F.·Gaus, K.·Lee, L. K.
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Abstract

Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

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Baker, M., Nieves, D. J., Hilzenrat, G., Berengut, J. F., Gaus, K., Lee, L. K.. 2019-01-21. Stoichiometric quantification of spatially dense assemblies with qPAINT. https://doi.org/10.1101/525345

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