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bioRxiv · 10.1101/524801

Nanoscopic Stoichiometry and Single-Molecule Counting

Nino, D.·Djayakarsana, D.·Milstein, J. N.
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Abstract

Single-molecule localization microscopy (SMLM) has the potential to revolutionize proteomic and genomic analyses by providing information on the number and stoichiometry of proteins or nucleic acids aggregating at spatial scales below the diffraction limit of light. Here we present a method for molecular counting with SMLM built upon the exponentially distributed blinking statistics of photoswitchable fluorophores, with a focus on organic dyes. We provide a practical guide to molecular counting, highlighting many of the challenges and pitfalls, by benchmarking the method on fluorescently labeled, surface mounted DNA origami grids. The accuracy of the results illustrates SMLMs utility for optical -omics analysis.

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Nino, D., Djayakarsana, D., Milstein, J. N.. 2019-01-18. Nanoscopic Stoichiometry and Single-Molecule Counting. https://doi.org/10.1101/524801

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