A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B

Brown, W.; Law, E.; Carpenter, M.; Argyris, P.; Levin-Klein, R.; Ranum, A.; Molan, A.; Forster, C.; Anderson, B.; Lackey, L.; Harris, R.

doi:10.1101/513341

bioRxiv · 10.1101/513341

A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B

Brown, W.·Law, E.·Carpenter, M.·Argyris, P.·Levin-Klein, R.·Ranum, A.·Molan, A.·Forster, C.·Anderson, B.·Lackey, L.·Harris, R.

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Abstract

The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B through a series of immunoblot, immunofluorescence microscopy, and immunohistochemistry experiments. Positive results indicate that one mAb, 5210-87-13, will be useful for fundamental research in virology and cancer biology and that immunohistochemical quantification of A3B enzyme levels may constitute a clinical biomarker for tumor evolvability and differential treatment.

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Brown, W., Law, E., Carpenter, M., Argyris, P., Levin-Klein, R., Ranum, A., Molan, A., Forster, C., Anderson, B., Lackey, L., Harris, R.. 2019-01-07. A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B. https://doi.org/10.1101/513341

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