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bioRxiv · 10.1101/513333

Contesting the evidence for -1 frameshifting in immune-functioning C-C chemokine receptor 5 (CCR5) - the HIV-1 co-receptor

Khan, Y. A.·Loughran, G.·Atkins, J. F.
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Abstract

During the decoding of a subset of mRNAs a proportion of ribosomes productively shift to the -1 reading frame at a specific, slippage-prone site1. While the great majority of occurrences of the \"Programmed -1 Ribosomal Frameshifting\" (-1 PRF) involve viruses2, other mobile elements or retroelements1, a dramatic instance of utilized human cellular -1 frameshifting in a non-retroelement derived mRNA has been reported. The mRNA for which -1 frameshifting was claimed due to a stimulatory pseudoknot is that which encodes immune functioning C-C chemokine receptor 5 (CCR5), the HIV-1 co-receptor3. The publication ascribed the importance of the reporter CCR5 mRNA frameshifting directing translating ribosomes to a premature termination codon leading to mRNA destabilization via the nonsense-mediated decay pathway (NMD), rather than creating a C- ...

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Khan, Y. A., Loughran, G., Atkins, J. F.. 2019-01-08. Contesting the evidence for -1 frameshifting in immune-functioning C-C chemokine receptor 5 (CCR5) - the HIV-1 co-receptor. https://doi.org/10.1101/513333

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