Non-FG-Nups, ELYS and Nup35, coordinate an ordered assembly of FG-Nups on the scaffold of the nuclear pore complex

Konishi, H. A.; Yoshimura, S. H.

doi:10.1101/506469

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bioRxiv · 10.1101/506469

Non-FG-Nups, ELYS and Nup35, coordinate an ordered assembly of FG-Nups on the scaffold of the nuclear pore complex

Konishi, H. A.·Yoshimura, S. H.

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Abstract

In this study, we examined how channel-forming subunits of the nuclear pore complex (NPC) are assembled into a selective channel within a highly structured scaffold ring during post-mitotic assembly. We focused on non-structured domains of the scaffold Nups and performed in vitro self-assembled particle assays with those derived from channel-forming FG-Nups. We found that non-structured domains of ELYS and Nup35N interacted with channel-forming FG-Nups to form a self-assembled particle. Sequential addition of FG-Nups into the scaffold particle revealed that ELYS, which initiates post-mitotic NPC reassembly, interacts with early assembling FG-Nups (Nups98 and 153) but not middle stage-assembling FG-Nups (Nups58 and 62). Nup35, which assembles between the early and middle stages, facilitated the assembly of Nup62 into the early assembling Nups both in vitro and in vivo. These results demonstrate that ELYS and Nup35 have a role of facilitator in the ordered assembly of channel-forming FG-Nups during mitosis.

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Konishi, H. A., Yoshimura, S. H.. 2018-12-26. Non-FG-Nups, ELYS and Nup35, coordinate an ordered assembly of FG-Nups on the scaffold of the nuclear pore complex. https://doi.org/10.1101/506469

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