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bioRxiv · 10.1101/505966

Fragmentation Through Polymerization (FTP): A New Method to Fragment DNA for Next-Generation Sequencing

Ignatov, K. B.·Blagodatskikh, K. A.·Shcherbo, D. S.·Kramarova, T.·Monakhova, Y. A.·Kramarov, V. M.
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Abstract

Fragmentation of DNA is the first and very important step in preparing nucleic acids for NGS. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is simple, robust and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for the direct use in NGS library construction, and allows to eliminate the need of an additional step of reparation of DNA ends.

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Ignatov, K. B., Blagodatskikh, K. A., Shcherbo, D. S., Kramarova, T., Monakhova, Y. A., Kramarov, V. M.. 2018-12-24. Fragmentation Through Polymerization (FTP): A New Method to Fragment DNA for Next-Generation Sequencing. https://doi.org/10.1101/505966

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