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bioRxiv · 10.1101/499640

SGK phosphorylates Cdc25 and Myt1 to trigger cyclin B-Cdk1 activation at the meiotic G2/M transition

Hiraoka, D.·Hosoda, E.·Chiba, K.·Kishimoto, T.
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Abstract

The kinase cyclin B-Cdk1 complex is a master regulator of M-phase in both mitosis and meiosis. At the G2/M transition, cyclin B-Cdk1 activation is initiated by a trigger that reverses the balance of activities between Cdc25 and Wee1/Myt1, and is further accelerated by autoregulatory loops. In somatic cell mitosis, this trigger was recently proposed to be the cyclin A-Cdk1/Plk1 axis. However, in the oocyte meiotic G2/M transition, in which hormonal stimuli induce cyclin B-Cdk1 activation, cyclin A-Cdk1 is non-essential and hence the trigger remains elusive. Here, we show that SGK directly phosphorylates Cdc25 and Myt1 to trigger cyclin B-Cdk1 activation in starfish oocytes. After hormonal stimulation of the meiotic G2/M transition, SGK is activated by cooperation between the G{beta}{gamma}-PI3K pathway and an unidentified pathway downstream of G{beta}{gamma}, called the atypical G{beta}{gamma} pathway. These findings identify the trigger in oocyte meiosis and provide insights into the role and activation of SGK.

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Hiraoka, D., Hosoda, E., Chiba, K., Kishimoto, T.. 2018-12-19. SGK phosphorylates Cdc25 and Myt1 to trigger cyclin B-Cdk1 activation at the meiotic G2/M transition. https://doi.org/10.1101/499640

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