The Hi-Culfite assay reveals relationships between chromatin contacts and DNA methylation state

Stamenova, E. K.; Durand, N.; Dudchenko, O.; Shamim, M. S.; Huang, S.-C.; Jiang, Y.; Bochkov, I. D.; Rao, S. S. P.; Lander, E. S.; Gnirke, A.; Aiden, E. L.

doi:10.1101/481283

bioRxiv · 10.1101/481283

The Hi-Culfite assay reveals relationships between chromatin contacts and DNA methylation state

Stamenova, E. K.·Durand, N.·Dudchenko, O.·Shamim, M. S.·Huang, S.-C.·Jiang, Y.·Bochkov, I. D.·Rao, S. S. P.·Lander, E. S.·Gnirke, A.·Aiden, E. L.

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Abstract

Hi-Culfite, a protocol combining Hi-C and whole-genome bisulfite sequencing (WGBS), determines chromatin contacts and DNA methylation simultaneously. Hi-Culfite also reveals relationships that cannot be seen when the two assays are performed separately. For instance, we show that loci associated with open chromatin exhibit context-sensitive methylation: when their spatial neighbors lie in closed chromatin, they are much more likely to be methylated.

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Stamenova, E. K., Durand, N., Dudchenko, O., Shamim, M. S., Huang, S.-C., Jiang, Y., Bochkov, I. D., Rao, S. S. P., Lander, E. S., Gnirke, A., Aiden, E. L.. 2018-11-29. The Hi-Culfite assay reveals relationships between chromatin contacts and DNA methylation state. https://doi.org/10.1101/481283

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