E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning

Donovan, D. A.; Crandall, J. G.; Banks, O. G.; Jensvold, Z. D.; McKnight, L. E.; McKnight, J. N.

doi:10.1101/480913

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bioRxiv · 10.1101/480913

E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning

Donovan, D. A.·Crandall, J. G.·Banks, O. G.·Jensvold, Z. D.·McKnight, L. E.·McKnight, J. N.

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Abstract

Regulation of chromatin structure is essential for controlling the access of DNA to factors that require association with specific DNA sequences. The ability to alter chromatin organization in a targeted manner would provide a mechanism for directly manipulating DNA-dependent processes and should provide a means to study direct consequences of chromatin structural changes. Here we describe the development and validation of engineered chromatin remodeling proteins (E-ChRPs) for inducing programmable changes in nucleosome positioning by design. We demonstrate that E-ChRPs function both in vivo and in vitro to specifically reposition target nucleosomes and entire nucleosomal arrays, and possess the ability to evict native DNA-binding proteins through their action. E-ChRPs can be designed with a range of targeting modalities, including the SpyCatcher and dCas9 moieties, resulting in high versatility and enabling diverse future applications. Thus, engineered chromatin remodeling proteins represent a simple and robust means to probe regulation of DNA-dependent processes in different chromatin contexts.

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Donovan, D. A., Crandall, J. G., Banks, O. G., Jensvold, Z. D., McKnight, L. E., McKnight, J. N.. 2018-11-29. E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning. https://doi.org/10.1101/480913

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