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bioRxiv · 10.1101/472787

Transient activation of the UPRER is an essential step in the acquisition of pluripotency during reprogramming

Simic, M.·Moehle, E.·Schinzel, R.·Lorbeer, F.·Jullie, D.·Halloran, J.·Heydari, K.·Sanchez, M.·Hockemeyer, D.·Dillin, A.
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Abstract

Somatic cells can be reprogrammed into pluripotent stem cells by the forced expression of the OCT4, SOX2, KLF4 and c-MYC transcription factors. This process requires the reshaping of not only epigenetic landscapes, but the global remodeling of cell identity, structure, and function including such basic processes of metabolism and organelle form and function. Cellular reprogramming is a stochastic process with only a marginally measureable fraction of cells successfully crossing these, and many other, cellular epitomes to acquire the fully pluripotent state. We hypothesize that this variation is due, in part, by variable regulation of the proteostasis network and its influence upon the protein folding environment within cells and their organelles upon the remodeling process. We find that the endoplasmic reticulum unfolded protein response (UPRER), the heat-shock response (HSR) and the mitochondrial unfolded protein response (UPRmt), which monitor and ensure the quality of the proteome of, respectively, the ER, the cytosol and the mitochondria during stress, are activated during cellular reprogramming. Particularly, we find that the UPRER is essential for reprograming, and ectopic, transient activation of the UPRER, either genetically or pharmacologically, enhances the success of cells to reach a pluripotent state. Finally, and most revealing, we find that stochastic activation of the UPRER can predict the reprogramming efficiency of naive cells. The results of these experiments indicate that the low efficiency and stochasticity of cellular reprogramming is partly the result of the inability to initiate a proper ER stress response for remodeling of the ER and its proteome during the reprogramming process. The results reported here display only one aspect of the proteostasis network and suggest that proper regulation of many more components of this network might be essential to acquire the pluripotent state.

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Simic, M., Moehle, E., Schinzel, R., Lorbeer, F., Jullie, D., Halloran, J., Heydari, K., Sanchez, M., Hockemeyer, D., Dillin, A.. 2018-11-17. Transient activation of the UPRER is an essential step in the acquisition of pluripotency during reprogramming. https://doi.org/10.1101/472787

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