Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/472316

Effects of Biogas Slurry on Fruit Economic Traits and Soil Nutrient of Camellia oleifera Abel

You, L.·Liu, H.·Yu, S.·Wang, C.·Zhou, Z.·Zhang, L.·Hu, D.
View original source ↗Export metadata ↓

Abstract

Soil nutrients play a principal role in Camellia oleifera Abel (oil-seed camellia) production. Camellia oleifera absorbs nutrients from surrounding soils and its production is highly influenced by nutrients or fertilization. In this study, we investigated the effects of biogas slurry applications on soil nutrients and economic traits of C. oleifera fruits. Five different amounts of fertilizing biogas slurry (0, 10, 20, 30, or 40 kg/plant/year from three applications per year) were applied to C. oleifera plants in 2015 and 2016. Rhizosphere soil nutrients and C. oleifera fruit economic traits (yield, seed rate, and oil yield)were measured. Fertilization with biogas slurryincreasedsoil organic matter, available nitrogen (N), phosphorus (P), and potassium (K) in both 2015 and 2016. Increases in soil available N, P, and Kwere largest at the highest slurry application rate and second largest at the second highest application rate. Fruit economic traits were maximized at the two highest application rates. Oil yield was correlated withsoil available P in 2015 and 2016, and soil organic matter in 2015. Fertilization with biogas slurry decreased saturated fatty acid content in fruit but had no effect on unsaturated fatty acid content. In conclusion, fertilization with biogas slurry increases rhizosphere soil nutrients and fruit economic traits of C. oleifera with the rates of at least 30 kg/plant/year having the most positive effects.

Source connections

Explore related subjects

plant biologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

You, L., Liu, H., Yu, S., Wang, C., Zhou, Z., Zhang, L., Hu, D.. 2018-11-16. Effects of Biogas Slurry on Fruit Economic Traits and Soil Nutrient of Camellia oleifera Abel. https://doi.org/10.1101/472316

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Anionic lipids stabilize etioplast prolamellar bodies and facilitate thylakoid biogenesis during etioplast-to-chloroplast differentiation

Etioplasts of dark-grown angiosperm seedlings contain prolamellar bodies (PLBs), lattice-like membranes that are quickly transformed into thylakoids upon illumination. Phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG) are major anionic lipids in these membranes, but their roles in etioplast-to-chloroplast differentiation remain unclear. We analyzed Arabidopsis thaliana mutants with reduced PG biosynthesis (pgp1-1), complete SQDG deficiency (sqd1), and the pgp1-1 sqd1 double mutant. Although pgp1-1 etioplasts showed a loosened PLB lattice when fixed under white light, they retained regular, wild-type-like lattices when fixed under infrared light, suggesting that their PLBs were more susceptible to light-induced disassembly. The rapid PLB disassembly in pgp1-1 upon illumination was accompanied by rapid conversion of dark-accumulated protochlorophyllide to chlorophyll and lamellar membrane formation, suggesting a link between membrane remodeling and chlorophyll formation. However, subsequent chlorophyll accumulation through de novo biosynthesis was retarded in illuminated pgp1-1 seedlings, whereas photosynthetic electron transport was quickly activated. Although sqd1 showed no obvious defects, pgp1-1 sqd1 exhibited more severe defects than pgp1-1, particularly in internal membrane formation, chlorophyll accumulation, photosynthetic activation, and photosynthetic gene expression. These findings suggest that PG is crucial for membrane-associated processes during etioplast-to-chloroplast differentiation, while SQDG may help maintain chloroplast development when PG availability is limited.

plant biology↗
bioRxiv2026

PHYRE, a Translated uORF, is Required for Phytochrome B Translation and Photomorphogenesis in Arabidopsis

Translational control allows cells to adjust protein output independently of transcription, yet the mRNA-encoded mechanisms that govern this regulatory layer remain poorly understood. Upstream open reading frames (uORFs) are among the most prevalent translational regulatory elements in eukaryotic genomes; however, characterised examples of activating uORFs that promote rather than inhibit downstream translation are exceedingly rare. Here we show that efficient expression of phytochrome B (phyB), the master red-light photoreceptor of Arabidopsis thaliana, requires translation of a short uORF in its own 5'UTR, which we name PHYTOCHROME REGULATOR (PHYRE). Loss of PHYRE function phenocopies phyB null mutants across germination, hypocotyl growth, leaf development, and flowering time. PHYRE activity depends on its encoded peptide sequence, and the spacing between PHYRE and the PHYB start codon is a critical determinant of downstream translation efficiency, consistent with a position-dependent reinitiation mechanism. PHYRE-like elements are conserved across eudicots, establishing uORF-mediated translational gating as a broadly maintained regulatory layer controlling a master photoreceptor.

plant biology↗
bioRxiv2026

The bHLH121-CAT2 module maintains root tip redox homeostasis to confer salt tolerance additively with the SOS pathway in Arabidopsis

Soil salinity inhibits plant growth, in part, by restricting the availability of essential mineral nutrients such as iron (Fe). Although Fe deficiency frequently coincides with salt stress in the field, the molecular mechanisms integrating Fe homeostasis with salt tolerance remain largely unknown. Here, we identify the Fe deficiency response regulator bHLH121 as a key coordinator of salt tolerance in Arabidopsis. Loss-of-function mutants of bHLH121 exhibit pronounced hypersensitivity to salt stress, with severely impaired primary root elongation and reduced seedling biomass. Transcriptomic and histochemical analyses reveal that bHLH121 is required to maintain reactive oxygen species (ROS) homeostasis specifically in root tips under salt stress. We further demonstrate that CATALASE 2 (CAT2) is a direct downstream target of bHLH121, and that CAT2 overexpression fully rescues the salt hypersensitivity of the bhlh121 mutant. Importantly, this bHLH121-CAT2 module operates independently of the classical SOS pathway, as sos2 bhlh121 double mutants display additive salt sensitivity. Together, our findings uncover a novel regulatory module in which bHLH121 governs root tip redox homeostasis through CAT2 to confer salt tolerance, providing a direct mechanistic link between the Fe regulatory network and abiotic stress resilience in plants.

plant biology↗