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bioRxiv · 10.1101/471771

NDV-3A vaccination protects mice from Candida albicans biofilm infection

Alqarihi, A.·Singh, S.·Edwards, J. E.·Ibrahim, A. S.·Uppuluri, P.
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Abstract

NDV-3A, a novel fungal vaccine undergoing clinical trials, contains a recombinant version of the Candida albicans rAls3 N-terminus protein (rAls3p-N) in aluminum hydroxide. In a Phase 1b/2a clinical trial, NDV-3A protected women from recurrent vulvovaginal candidiasis. Here, we reveal that active immunization in mice with NDV-3A induces high titers of anti-rAls3p-N antibodies that interfere with C. albicans ability to adhere to and invade endothelial cells, and form biofilm in vitro. The anti-rAls3p-N antibodies also protect against C. albicans biofilm formation in a central venous catheter mouse model. Finally, anti-rAls3p-N antibodies significantly inhibit yeast dispersal from the hyphal layers of biofilms. Overall these preclinical studies suggest that NDV-3A may serve as an immunotherapeutic strategy for prevention of infections on indwelling medical devices.

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Alqarihi, A., Singh, S., Edwards, J. E., Ibrahim, A. S., Uppuluri, P.. 2018-11-19. NDV-3A vaccination protects mice from Candida albicans biofilm infection. https://doi.org/10.1101/471771

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