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bioRxiv · 10.1101/465930

Enhanced cell-cell contact stability upon Fibroblast Growth factor Receptor/N-cadherin cross-talk

Nguyen, T.·Duchesne, L.·Bogetto, N.·Fernig, D.·Murade, C.·Ladoux, B.·Mege, R.-M.
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Abstract

N-cadherin adhesion has been reported to enhance cancer and neuronal cell migration either by mediating actomyosin-based force transduction or initiating Fibroblast Growth Factor Receptor (FGFR)-dependent biochemical signalling. Here we show that FGFR1 reduces N-cadherin-mediated cell migration. Both proteins are co-stabilised at cell-cell contacts through direct interaction. As a consequence, cell adhesion is strengthened, limiting the migration of cells on N-cadherin. Both the inhibition of migration and the stabilisation of cell adhesions require the FGFR activity stimulated by N-cadherin engagement. FGFR1 stabilises N-cadherin at the cell membrane through a pathway involving Src and p120. Moreover, FGFR1 stimulates the anchoring of N-cadherin to actin. We found that the migratory behaviour of cells depends on an optimum balance between FGFR-regulated N-cadherin adhesion and actin dynamics. Based on these findings we propose a positive feedback loop between N-cadherin and FGFR at adhesion sites limiting N-cadherin-based single cell migration.

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Nguyen, T., Duchesne, L., Bogetto, N., Fernig, D., Murade, C., Ladoux, B., Mege, R.-M.. 2018-11-08. Enhanced cell-cell contact stability upon Fibroblast Growth factor Receptor/N-cadherin cross-talk. https://doi.org/10.1101/465930

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