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bioRxiv · 10.1101/465492

A stable, high refractive index, switching buffer for super-resolution imaging

Hartwich, T. M.·Chung, K. K.·Schroeder, L.·Bewersdorf, J.·Soeller, C.·Baddeley, D.
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Abstract

dSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

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Hartwich, T. M., Chung, K. K., Schroeder, L., Bewersdorf, J., Soeller, C., Baddeley, D.. 2018-11-08. A stable, high refractive index, switching buffer for super-resolution imaging. https://doi.org/10.1101/465492

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