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bioRxiv · 10.1101/459594

Minimization of cross-talk between Spindle Assembly Checkpoint silencing and error correction

Roy, B.·Verma, V.·Sim, J.·Fontan, A.·Joglekar, A. P.
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Abstract

Accurate chromosome segregation during cell division requires that the pair of sister kinetochores on each chromosome attach to microtubules originating from opposite spindle poles. This is ensured by the combined action of the Spindle Assembly Checkpoint (SAC), which detects unattached kinetochores, and an error correction mechanism that destabilizes incorrect attachment of both sister kinetochores to the same spindle pole. These processes are downregulated by Protein Phosphatase 1 (PP1), which both silences the SAC and stabilizes kinetochore-microtubule attachments. We find that this dual PP1 role can be problematic: if PP1 is recruited to the kinetochore for SAC silencing prior to chromosome biorientation, it interferes with error correction. We show that to mitigate this cross-talk, the yeast kinetochore uses independent PP1 sources to stabilize correct attachments and to silence the SAC, and also delays the recruitment of PP1 for SAC silencing. Consequently, chromosome biorientation precedes SAC silencing ensuring accurate chromosome segregation.

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Roy, B., Verma, V., Sim, J., Fontan, A., Joglekar, A. P.. 2018-11-01. Minimization of cross-talk between Spindle Assembly Checkpoint silencing and error correction. https://doi.org/10.1101/459594

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