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bioRxiv · 10.1101/445973

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing

Viita, T.·Kyheröinen, S.·Prajapati, B.·Virtanen, J.·Varjosalo, M.·Vartiainen, M.
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Abstract

In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanisms by which actin operates in the nucleus remain poorly understood. Here we have used two complementary mass spectrometry (MS) techniques, AP-MS and BioID-MS, to identify binding partners for nuclear actin. Common high-confidence interactions highlight the role of actin in chromatin remodeling complexes and identify the hATAC histone modifier as a novel actin-containing nuclear complex. Further analysis demonstrates that actin binds directly to the hATAC subunit KAT14, and modulates its histone acetyl transferase activity in vitro and in cells. BioID-MS, which can detect also transient interactions, links actin to several steps of transcription as well as to RNA processing. Alterations in nuclear actin levels disturb alternative exon skipping of the SMN2 minigene, suggesting also a functional role for actin in RNA splicing. This interactome analysis thus identifies both novel direct binding partners and functional roles for nuclear actin, as well as forms a platform for further mechanistic studies on how actin operates during essential nuclear processes.

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Viita, T., Kyheröinen, S., Prajapati, B., Virtanen, J., Varjosalo, M., Vartiainen, M.. 2018-10-17. Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing. https://doi.org/10.1101/445973

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