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bioRxiv · 10.1101/443804

Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16

Zhang, X.·Crowley, V. M.·Wucherpfennig, T. G.·Dix, M. M.·Cravatt, B. F.
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Abstract

Ligand-dependent protein degradation has emerged as a compelling strategy to pharmacologically control the protein content of cells. So far, only a limited number of E3 ligases have been found to support this process. Here, we use a chemical proteomic strategy to discover that DCAF16 - a poorly characterized substrate recognition component of CUL4-DDB1 E3 ubiquitin ligases - promotes nuclear-restricted protein degradation upon modification by cysteine-directed heterobifunctional electrophilic compounds.

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Zhang, X., Crowley, V. M., Wucherpfennig, T. G., Dix, M. M., Cravatt, B. F.. 2018-10-15. Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16. https://doi.org/10.1101/443804

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