Immunoinformatics Prediction of Epitope Based Peptide Vaccine Against Madurella mycetomatis Translationally Controlled Tumor Protein

Bolis, S.; Osman, W.; Anwar, M.; Abdelmagid, M.; Ahmed, E.; Abaker, M.; Abdelwahab, W.; Hashim, S.; Abubakralsiddig, A.; Algaali, A.; Hashim, O.; Hassan, M.

doi:10.1101/441881

bioRxiv · 10.1101/441881

Immunoinformatics Prediction of Epitope Based Peptide Vaccine Against Madurella mycetomatis Translationally Controlled Tumor Protein

Bolis, S.·Osman, W.·Anwar, M.·Abdelmagid, M.·Ahmed, E.·Abaker, M.·Abdelwahab, W.·Hashim, S.·Abubakralsiddig, A.·Algaali, A.·Hashim, O.·Hassan, M.

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Abstract

BackgroundMadurella. mycetomatis is most common causative agent of mycetoma in Sudan and worldwide. No vaccines are available till now so design of effective vaccine is essential as protection tool. Peptide vaccine can overcome the common side effects of the conventional vaccines. The aim of this study was to design peptide based vaccine for M.Mycetomatis Translationally Controlled Tumor Protein (TCTP) using immunoinformatics tools.\n\nMaterials and methodsTCTP sequences were retrieved from NCBI and then processed using BioEdit program to determine conserved regions and different immunoinformatics tools from IEDB. Population coverage analysis was performed for the most promising epitopes. Homology modelling was performed to show their structural positions in TCTP. Protein analysis was done using Expasy (ProtParamsotware).\n\nResults and conclusionFour epitopes passed the Bepipred, Emini, Kolaskar and Tongaonkar tools. 111 epitopes were predicted to interact with MHCI alleles with IC50 < 500 nM, three of them were most promising. 274 predicted epitopes were interacted with MHCII alleles with IC50 < 100 nM, four of them were most promising. The epitope (YMKSVKKAL) was the most promising one concerning its binding with MHCI alleles, while (FRLQSTSFD) was the most promising for MHC II. The epitope (YLKAYMKSV) is shared betweenMHC I and II. For the population coverage of M. Mycetomatis TCTP vaccine Sudan (90.39%) had the highest percentage for MHC I. This is the first computational vaccinology study conducted in mycetoma caused by M. Mycetomatis using TCTP.

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Bolis, S., Osman, W., Anwar, M., Abdelmagid, M., Ahmed, E., Abaker, M., Abdelwahab, W., Hashim, S., Abubakralsiddig, A., Algaali, A., Hashim, O., Hassan, M.. 2018-10-13. Immunoinformatics Prediction of Epitope Based Peptide Vaccine Against Madurella mycetomatis Translationally Controlled Tumor Protein. https://doi.org/10.1101/441881

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