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bioRxiv · 10.1101/441469

Dissociation rate compensation mechanism for budding yeast pioneer transcription factors

Donovan, B. T.·Chen, H.·Jipa, C.·Bai, L.·Poirier, M. G.
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Abstract

Nucleosomes restrict the occupancy of most transcription factors (TF) by reducing binding and accelerating dissociation, while a small group of TFs have high affinities to nucleosome-embedded sites and facilitate nucleosome displacement. To mechanistically understand this process, we investigated two S. cerevisiae TFs, Reb1 and Cbf1. We show these factors bind their sites within nucleosomes with similar affinities to naked DNA, trapping a partially unwrapped nucleosome without histone eviction. Both the binding and dissociation rates of Reb1 and Cbf1 are significantly slower at the nucleosomal sites relative to DNA, demonstrating that the high affinities are achieved by increasing the dwell time on nucleosomes to compensate for reduced binding. Reb1 also shows slow migration rate in the yeast nuclei. These properties are similar to human pioneer factors (PFs), suggesting the mechanism of nucleosome targeting is conserved from yeast to human.

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Donovan, B. T., Chen, H., Jipa, C., Bai, L., Poirier, M. G.. 2018-10-12. Dissociation rate compensation mechanism for budding yeast pioneer transcription factors. https://doi.org/10.1101/441469

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