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bioRxiv · 10.1101/441444

Antigen receptor locus dynamics is orchestrated near the sol-gel phase transition to enforce stepwise VDJ gene rearrangement

Khanna, N.·Zhang, J.·Dudko, O.·Murre, C.
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Abstract

Diverse antibody repertoires are generated through remote genomic interactions involving immunoglobulin variable (VH), diversity (DH) and joining (JH) gene segments. How such interactions are orchestrated remains unknown. We developed a novel strategy to track VH-DHJH motion and interactions in live B-lymphocytes. We found that VH and DHJH segments were trapped in configurations that only allowed constrained local motion, such that spatially proximal VH and DHJH segments remained in proximity, whereas spatially remote segments explored their immediate neighborhood while remaining remote. Comparison of experimental and simulated data revealed that such a highly constrained motion was imposed by a network of cross-linked chromatin chains characteristic of a gel phase, yet it was poised near the sol phase, a solution of independent chromatin chains. We propose that epigenetically induced gel droplets and the proximity to the sol-gel phase transition constitute the mechanism that orchestrates ordered VDJ rearrangement.

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Khanna, N., Zhang, J., Dudko, O., Murre, C.. 2018-10-12. Antigen receptor locus dynamics is orchestrated near the sol-gel phase transition to enforce stepwise VDJ gene rearrangement. https://doi.org/10.1101/441444

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