KPNA2 promotes cellular proliferation and inhibits apoptosis in the Saos-2 osteosarcoma cell line

Wei, D.; Ge, Q.; Sun, X.; Zhang, L.; Li, J.; Chen, C.; lv, z.; Dinglu WeiQiaofeng GeXiaojuan SunLong ZhangJia LiChenglong ChenZhi Lv,

doi:10.1101/436428

bioRxiv · 10.1101/436428

KPNA2 promotes cellular proliferation and inhibits apoptosis in the Saos-2 osteosarcoma cell line

Wei, D.·Ge, Q.·Sun, X.·Zhang, L.·Li, J.·Chen, C.·lv, z.·Dinglu WeiQiaofeng GeXiaojuan SunLong ZhangJia LiChenglong ChenZhi Lv,

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Abstract

Karyopherin 2 plays a critical role in tumorigenesis and tumor progression. However, nothing is currently known about the effects of KPNA2 on osteosarcomas. This study aimed to investigate differential KPNA2 protein and mRNA expression in human osteosarcoma tumor cells and normal bone tissue. We also sought to determine whether KPNA2 can influence the proliferation and apoptosis of the Saos-2 osteosarcoma cell line. Immunohistochemistry (IHC) was used to investigate KPNA2 protein expression. Real-time quantitative PCR (qPCR) was used to detect levels of KPNA2 mRNA expression, and lentivirus-mediated short-hairpin RNAs (shRNAs) were used to knock down KPNA2 expression in Saos-2 cells. The MTT assay and multiparametric high-content screening (HCS) were used to measure cell proliferation and growth, respectively. Flow cytometry was conducted to detect cell cycle distribution and apoptosis. The results revealed significantly higher KPNA2 expression levels in osteosarcoma tissues than in normal bone tissues; furthermore, KPNA2 mRNA was also highly expressed in three osteosarcoma cell lines. After transducing Saos-2 cells with KPNA2-shRNA lentivirus, the proliferative rate was notably decreased compared to that of the negative control (NC) lentivirus group (P<0.05). Flow cytometry results indicated that KPNA2 may arrest cell cycle progression and regulate the growth of these cells. The results for apoptosis indicated an apoptotic rate of 13.38{+/-}0.48% in KPNA2-shRNA cells, which was significantly higher than the rate for cells in the control group (5.13 {+/-}0.33%). Therefore, this study showed that KPNA2 is highly expressed in osteosarcoma tissues and that reduced KPNA2mRNA levels inhibited proliferation and promoted apoptosis in an osteosarcoma cell line.

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Wei, D., Ge, Q., Sun, X., Zhang, L., Li, J., Chen, C., lv, z., Dinglu WeiQiaofeng GeXiaojuan SunLong ZhangJia LiChenglong ChenZhi Lv,. 2018-10-05. KPNA2 promotes cellular proliferation and inhibits apoptosis in the Saos-2 osteosarcoma cell line. https://doi.org/10.1101/436428

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