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bioRxiv · 10.1101/432740

Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor

Abstract

The pivotal task of the immune system is to distinguish between self and foreign antigens. The kinetic proofreading model (KPR) proposes that T cells discriminate self from foreign ligands by the different ligand binding half-lives to the T cell receptor (TCR). It is challenging to test KPR as the available experimental systems fall short of only altering the binding half-lives and keeping other parameters of the ligand-TCR interaction unchanged. We engineered an optogenetic system using the plant photoreceptor phytochrome B to selectively control the dynamics of ligand binding to the TCR by light. Combining experiments with mathematical modeling we find that the ligand-TCR interaction half-life is the decisive factor for activating downstream TCR signaling, substantiating the KPR hypothesis.\n\nOne Sentence SummaryThe half-life of the ligand-T cell receptor complex determines T cell activation.

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BibTeXRIS

Yousefi, O. S., Guenther, M., Hoerner, M., Chalupsky, J., Wess, M., Brandl, S. M., Smith, R. W., Fleck, C., Kunkel, T., Zurbriggen, M. D., Hoefer, T., Weber, W., Schamel, W. W. A.. 2018-10-01. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. https://doi.org/10.1101/432740

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