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bioRxiv · 10.1101/427468

Universal Light-Sheet Generation with Field Synthesis

Chang, B.-J.·Kittisopikul, M.·Dean, K. M.·Roudot, P.·Welf, E.·Fiolka, R. P.
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Abstract

We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a drastically simplified optical design, higher light-throughput, simultaneous multicolor illumination, and a 100% spatial duty cycle, thereby providing uncompromised biological imaging with decreased rates of photobleaching.

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Chang, B.-J., Kittisopikul, M., Dean, K. M., Roudot, P., Welf, E., Fiolka, R. P.. 2018-09-26. Universal Light-Sheet Generation with Field Synthesis. https://doi.org/10.1101/427468

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