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bioRxiv · 10.1101/426973

beditor: A computational workflow for designing libraries of guide RNAs for CRISPR base editing

Dandage, R.·Despres, P. C.·Yachie, N.·Landry, C. R.
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Abstract

CRISPR-mediated base editors have opened unique avenues for scar-free genome-wide mutagenesis. Here, we describe a comprehensive computational workflow called beditor that can be broadly adapted for designing guide RNA libraries with a range of CRISPR-mediated base editors, PAM recognition sequences and genomes of many species. Additionally, in order to assist users in selecting the best sets of guide RNAs for their experiments, a priori estimates, called beditor scores are calculated. These beditor scores are intended to select guide RNAs that conform to requirements for optimal base editing: editable base falls within maximum activity window of the CRISPR-mediated base editor and produces non-confounding mutational effects with minimal off-target effects. We demonstrate the utility of the software by designing guide RNAs for base-editing mediated edition of thousands of clinically important human disease mutations.

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Dandage, R., Despres, P. C., Yachie, N., Landry, C. R.. 2018-09-25. beditor: A computational workflow for designing libraries of guide RNAs for CRISPR base editing. https://doi.org/10.1101/426973

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