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bioRxiv · 10.1101/426957

High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

Gustafsson, C.·De Paepe, A.·Schmidl, C.·Mansson, R.
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Abstract

Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Here, we present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

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Gustafsson, C., De Paepe, A., Schmidl, C., Mansson, R.. 2018-09-27. High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers. https://doi.org/10.1101/426957

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