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bioRxiv · 10.1101/415190

Biophysical mechanisms in the mammalian respiratory oscillator re-examined with a new data-driven computational model

Phillips, R.·John, T. T.·Koizumi, H.·Molkov, Y. I.·Smith, J. C.
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Abstract

An autorhythmic population of excitatory neurons in the brainstem pre-Botzinger complex is a critical component of the mammalian respiratory oscillator. Two intrinsic neuronal biophysical mechanisms--a persistent sodium current (INaP) and a calcium-activated non-selective cationic current (ICAN)--were proposed to individually or in combination generate cellular-and circuit-level oscillations, but their roles are debated without resolution. We re-examined these roles with a new computational model of an excitatory population with randomly distributed INaP and ICAN conductances and synaptic connections. This model robustly reproduces experimental data showing contrary to previous hypotheses, rhythm generation is independent of ICAN activation, which instead determines population activity amplitude. The novel insight is that this occurs when ICAN is primarily activated by neuronal calcium fluxes driven by synaptic mechanisms. Rhythm depends critically on INaP in a subpopulation forming the rhythmogenic kernel. The model explains how the rhythm and amplitude of respiratory oscillations involve distinct biophysical mechanisms.

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Phillips, R., John, T. T., Koizumi, H., Molkov, Y. I., Smith, J. C.. 2018-09-12. Biophysical mechanisms in the mammalian respiratory oscillator re-examined with a new data-driven computational model. https://doi.org/10.1101/415190

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