RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction point

Lee, J.; Kim, D.; Jang, J.; Park, T.; Lee, Y.; Chi, X.-Z.; Park, I. Y.; Hyun, J.; Ito, Y.; Bae, S.-C.

doi:10.1101/409227

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bioRxiv · 10.1101/409227

RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction point

Lee, J.·Kim, D.·Jang, J.·Park, T.·Lee, Y.·Chi, X.-Z.·Park, I. Y.·Hyun, J.·Ito, Y.·Bae, S.-C.

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Abstract

The cellular decision regarding whether to undergo proliferation or death is made at the restriction (R)-point, which is disrupted in nearly all tumors. The identity of the molecular mechanisms that govern the R-point decision is one of the fundamental issues in cell biology. We found that early after mitogenic stimulation, RUNX3 bound to its target loci, where it opened chromatin structure by sequential recruitment of Trithorax group proteins and cell-cycle regulators to drive cells to the R-point. Soon after, RUNX3 closed these loci by recruiting Polycomb repressor complexes, causing the cell to pass through the R-point toward S phase. If the RAS signal was constitutively activated, RUNX3 inhibited cell cycle progression by maintaining R-point-associated genes in an open structure. Our results identify RUNX3 as a pioneer factor for the R-point and reveal the molecular mechanisms by which appropriate chromatin modifiers are selectively recruited to target loci for appropriate R-point decisions.

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Lee, J., Kim, D., Jang, J., Park, T., Lee, Y., Chi, X.-Z., Park, I. Y., Hyun, J., Ito, Y., Bae, S.-C.. 2018-09-07. RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction point. https://doi.org/10.1101/409227

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