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bioRxiv · 10.1101/408575

PBRM1 regulates stress response in epithelial cells

Porter, E. G.·Dhiman, A.·Chowdhury, B.·Carter, B. C.·Lin, H.·Stewart, J. C.·Kazemian, M.·Wendt, M. K.·Dykhuizen, E.
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Abstract

Polybromo1 (PBRM1) is a chromatin remodeler subunit highly mutated in cancer, particularly renal clear cell carcinoma. PBRM1 is a member of the SWI/SNF subcomplex, PBAF (PBRM1-Brg1/Brm Associated Factors) and is characterized by six tandem bromodomains. Here we establish a role for PBRM1 in epithelial cell maintenance through the expression of genes involved in cell adhesion, metabolism, stress response, and apoptosis. In support of a general role for PBRM1 in stress response and apoptosis, we observe that loss of PBRM1 results in an increase in reactive oxygen species generation and a decrease in cellular viability under stress conditions. We find that loss of PBRM1 promotes cell growth under favorable conditions but is required for cell survival under conditions of cellular stress.

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Porter, E. G., Dhiman, A., Chowdhury, B., Carter, B. C., Lin, H., Stewart, J. C., Kazemian, M., Wendt, M. K., Dykhuizen, E.. 2018-09-04. PBRM1 regulates stress response in epithelial cells. https://doi.org/10.1101/408575

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