Haplotype Analysis of the TRB Locus by TCRB Repertoire Sequencing

Looney, T.; Duose, D. Y.; Lowman, G.; Linch, E.; Hajjar, J.; Topacio-Hall, D.; Xu, M.; Zheng, J.; Alshawa, A.; Tapia, C.; Stephen, B. A.; Wang, L.; Meric-Bernstam, F.; Miller, L.; Glavin, A.; Lin, L.; Gong, J.; Conroy, J.; Morrison, C.; Hyland, F.; Naing, A.

doi:10.1101/406157

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bioRxiv · 10.1101/406157

Haplotype Analysis of the TRB Locus by TCRB Repertoire Sequencing

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Abstract

Polymorphism within the T cell receptor beta variable gene (TRBV) has been implicated in autoimmune disease and immuneCrelated adverse events (IRAEs) during immunotherapy. Previous efforts to evaluate TRBV polymorphism by whole genome sequencing (WGS) have been hampered by the repetitive nature of the TCRB locus. We present a novel longCamplicon TCRB repertoire sequencing approach to evaluate TRBV polymorphism from peripheral blood, which we use to identify TRBV allele haplotypes in 81 Caucasians.

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Looney, T., Duose, D. Y., Lowman, G., Linch, E., Hajjar, J., Topacio-Hall, D., Xu, M., Zheng, J., Alshawa, A., Tapia, C., Stephen, B. A., Wang, L., Meric-Bernstam, F., Miller, L., Glavin, A., Lin, L., Gong, J., Conroy, J., Morrison, C., Hyland, F., Naing, A.. 2018-09-07. Haplotype Analysis of the TRB Locus by TCRB Repertoire Sequencing. https://doi.org/10.1101/406157

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