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bioRxiv · 10.1101/402966

High-throughput single-cell transcriptome profiling of plant cell types

Shulse, C. N.·Cole, B. J.·Turco, G. M.·Zhu, Y.·Brady, S. M.·Dickel, D. E.
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Abstract

Single-cell transcriptome analysis of heterogeneous tissues can provide high-resolution windows into the genomic basis and spatiotemporal dynamics of developmental processes. Here we demonstrate the feasibility of high-throughput single-cell RNA sequencing of plant tissue using the Drop-seq approach. Profiling of >4,000 individual cells from the Arabidopsis root provides transcriptomes and marker genes for a diversity of cell types and illuminates the gene expression changes that occur across endodermis development.

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Shulse, C. N., Cole, B. J., Turco, G. M., Zhu, Y., Brady, S. M., Dickel, D. E.. 2018-08-29. High-throughput single-cell transcriptome profiling of plant cell types. https://doi.org/10.1101/402966

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