Establishment of baseline sensitivity of Rhizoctonia solani to thifluzamide in corn and its field application

shang, d.; Yao, C.; He, F.; Li, X.; Zhang, J.; Jiang, X.; Sun, X.; Sun, S.; Tan, H.; Chentao Yao, Falin He, Xiao Sun, Shiang Sun, Haili Tan, Xiangdong Li, Jiwang Zhang, Xingyin Jiang,

doi:10.1101/402529

bioRxiv · 10.1101/402529

Establishment of baseline sensitivity of Rhizoctonia solani to thifluzamide in corn and its field application

shang, d.·Yao, C.·He, F.·Li, X.·Zhang, J.·Jiang, X.·Sun, X.·Sun, S.·Tan, H.·Chentao Yao, Falin He, Xiao Sun, Shiang Sun, Haili Tan, Xiangdong Li, Jiwang Zhang, Xingyin Jiang,

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Abstract

In recent years, banded leaf sheath blight in corn has become an important disease in corn that seriously affects quality and yield. This paper aims to evaluate the sensitivity of Rhizoctonia solani to thifluzamide in corn, to clarify the effect of seed coating using a thifluzamide suspension agent on safety and physiological indicators and to determine banded leaf sheath blight in corn control effectiveness in the field, thereby providing a basis for the application of thifluzamide suspension agent as a seed coating. In this study, the thifluzamide sensitivity of 102 strains of R. solani in corn in different regions of Shandong was determined using the mycelial growth rate method, and the average half-maximal effective concentration value (EC50) was 0.086{+/-}0.004 g/mL. The sensitivity was consistent with a continuous and skewed normal distribution, and the sensitivity distribution frequency exhibited a continuous, unimodal curve, indicating that thifluzamide had strong inhibitory activity on the mycelial growth of R. solani in corn. The impacts of using a thifluzamide suspension agent for seed coating on safety and physiological indicators as well as the control effect in corn were evaluated by combining seed coating, an indoor pot test, and a field trial. The root activities under 24 g a.i. 100 kg-1 seed and 12 g a.i. 100 kg-1 seed were found to increase by 78.01% and 77.40%, respectively, compared with that under the blank control; the chlorophyll content of corn increased most significantly at a dosage of 24 g a.i. 100 kg-1, which was a 32.32% increase compared to the blank control. Thifluzamide (FS) could significantly increase the hundred-grain weight of corn and the per-plot yield. Among the examined dosages, 24 g a.i. 100 kg-1 seed had the most significant treatment effect, with the hundred-grain weight increasing by 12.47% and the yield rate increasing by 15.72% compared to the control in 2016, Simultaneously, the hundred-grain weight increasing by 13.44% and the yield rate increasing by 14.11% compared to the control in 2017. Three dosages of 24% thifluzamide (FS) increased the emergence rate and seedling growth of corn to varying extents. The field control effectiveness against banded leaf sheath blight in corn was best at the dosage of 24 g a.i. 100 kg-1 seed for seed dressing with thifluzamide (FS); in 2016 and 2017, the control effects in the small bell stage, large bell stage, tasseling and pollen-shedding stage, silking stage, milk-ripening stage, and wax-ripening stage were 100%, 66.73%, 52.8%, 67.81%, 68.48%, and 62.68% (2016), respectively, and 74.97%, 63.17%, 50.90%, 53.60%, 61.42%, and 55.88% (2017). These results indicated that thifluzamide had enormous potential for controlling banded leaf sheath blight in corn.

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shang, d., Yao, C., He, F., Li, X., Zhang, J., Jiang, X., Sun, X., Sun, S., Tan, H., Chentao Yao, Falin He, Xiao Sun, Shiang Sun, Haili Tan, Xiangdong Li, Jiwang Zhang, Xingyin Jiang,. 2018-08-28. Establishment of baseline sensitivity of Rhizoctonia solani to thifluzamide in corn and its field application. https://doi.org/10.1101/402529

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