Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors

Voyvodic, P. L.; Pandi, A.; Koch, M.; Faulon, J.-L.; Bonnet, J.

doi:10.1101/397315

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bioRxiv · 10.1101/397315

Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors

Voyvodic, P. L.·Pandi, A.·Koch, M.·Faulon, J.-L.·Bonnet, J.

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Abstract

Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a framework to expand the range of molecules detectable by cell-free biosensors by combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors are highly-sensitive and have a fast response and high-dynamic range. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.

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Voyvodic, P. L., Pandi, A., Koch, M., Faulon, J.-L., Bonnet, J.. 2018-08-21. Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space of Cell-Free Biosensors. https://doi.org/10.1101/397315

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