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bioRxiv · 10.1101/390641

Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

Clarke, D.·Idris, A.·McMillan, N. A.
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Abstract

Liposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage room temperature (RT), 4{degrees}C or - 20{degrees}C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.

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Clarke, D., Idris, A., McMillan, N. A.. 2018-08-13. Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage. https://doi.org/10.1101/390641

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