Candidate genes under balancing selection in a plant bacterial pathogen

Abstract

Plant pathogens are under significant selective pressure by the plant host. Consequently, they are expected to have adapted to this condition or contribute to evading plant defenses. In order to acquire long-term fitness, plant bacterial pathogens are usually forced to maintain advantageous genetic diversity in populations. This strategy ensures that different alleles in the pathogens gene pool are maintained in a population at frequencies larger than expected under neutral evolution. This selective process, known as balancing selection, is the subject of this work in the context of a common plant bacterial pathogen. We performed a genome-wide scan of Ralstonia solanacearum, an aggressive plant bacterial pathogen that shows broad host range and causes a devastating disease called bacterial wilt. Using a sliding window approach, we analyzed 57 genomes from three phylotypes of R. solanacearum to detect signatures of balancing selection. A total of 161 windows showed extreme values in three summary statistics of population genetics: Tajimas D, Wattersons {theta} and Fu & Lis D*. We discarded any confounding effects due to demographic events by means of coalescent simulations of genetic data. The prospective windows correspond to 78 genes that map in any of the two main replicons of R. solanacearum. The candidate genes under balancing selection are related to primary metabolism (51.3%) or directly associated to virulence (48.7%), being involved in key functions targeted to dismantle plant defenses or to participate in critical stages in the pathogenic process. These genes are useful to understand and monitor the evolution of bacterial pathogen populations and emerge as potential candidates for future treatments to induce specific plant immune responses.