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bioRxiv · 10.1101/386201

An Evolutionarily Conserved piRNA-producing Locus Required for Male Mouse Fertility

Abstract

Pachytene piRNAs, which comprise >80% of small RNAs in the adult mouse testis, have been proposed to bind and regulate target RNAs like miRNAs, cleave targets like siRNAs, or lack biological function altogether. Although piRNA pathway protein mutants are male sterile, no biological function has been identified for any mammalian piRNA-producing locus. Here, we report that males lacking piRNAs from a conserved mouse pachytene piRNA locus on chromosome 6 (pi6) produce sperm with defects in capacitation and egg fertilization. Moreover, heterozygous embryos sired by pi6-/- fathers show reduced viability in utero. Molecular analyses suggest that pi6 piRNAs repress gene expression by cleaving mRNAs encoding proteins required for sperm function. pi6 also participates in a network of piRNA-piRNA precursor interactions that initiate piRNA production from a second piRNA locus on chromosome 10 as well as pi6 itself. Our data establish a direct role for pachytene piRNAs in spermiogenesis and embryo viability.\n\nHighlightsO_LINormal male mouse fertility and spermiogenesis require piRNAs from the pi6 locus\nC_LIO_LISperm capacitation and binding to the zona pellucida of the egg require pi6 piRNAs\nC_LIO_LIHeterozygous embryos sired by pi6-/- fathers show reduced viability in utero\nC_LIO_LIDefects in pi6 mutant sperm reflect changes in the abundance of specific mRNAs.\nC_LI

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Wu, P.-H., Fu, Y., Cecchini, K., Ozata, D. M., Weng, Z., Zamore, P. D.. 2018-08-07. An Evolutionarily Conserved piRNA-producing Locus Required for Male Mouse Fertility. https://doi.org/10.1101/386201

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