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bioRxiv · 10.1101/377630

Assessing sub-cellular resolution in spatial proteomics experiments

Gatto, L.·Breckels, L. M.·Lilley, K. S.
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Abstract

The sub-cellular localisation of a protein is vital in defining its function, and a proteins mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.

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Gatto, L., Breckels, L. M., Lilley, K. S.. 2018-07-25. Assessing sub-cellular resolution in spatial proteomics experiments. https://doi.org/10.1101/377630

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