An automated workflow for the discovery and docking simulation of the protein-protein complexes using in vivo chemical cross-linking

Svetlov, V.; Nudler, E.

doi:10.1101/373829

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bioRxiv · 10.1101/373829

An automated workflow for the discovery and docking simulation of the protein-protein complexes using in vivo chemical cross-linking

Svetlov, V.·Nudler, E.

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Abstract

Introduction Introduction Results Discussion Materials and Methods Competing interests Contributions Materials and Correspondence References Chemical cross(X)-link mapping assisted by mass spectrometry (XL-MS, also CXMS and CLMS) is a low-resolution hybrid method of structural biology, yielding a set of pairwise distance restraints between reactive solvent-accessible amino acids1-9. Most commonly used X-linkers at present belong to the class of amino-reactive homo-bifunctional NHS-esters, which act as protein proximity sensors, connecting predominantly Lys residues (as well as far less common and informative N-terminal amines)10-14. Main structural application of XL-MS to date i ...

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Svetlov, V., Nudler, E.. 2018-07-20. An automated workflow for the discovery and docking simulation of the protein-protein complexes using in vivo chemical cross-linking. https://doi.org/10.1101/373829

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