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bioRxiv · 10.1101/369983

The landscape of S100B+ and HLA-DR+ dendritic cell subsets in tonsils at the single cell level via high-parameter mapping

Bolognesi, M. M.·Bosisio, F. M.·Manzoni, M.·Schapiro, D.·Tagliabue, R.·Faretta, M.·Parravicini, C.·Haberman, A. M.·Cattoretti, G.
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Abstract

Dendritic cells (DC) (classic, plasmacytoid, inflammatory) are an intense focus of interest because of their role in inflammation, autoimmunity, vaccination and cancer. We present a tissue-based classification of human DC subsets in tonsils with a high-parameter (>40 markers) immunofluorescent approach, cell type-specific image segmentation and the use of bioinformatics platforms. Through this deep phenotypic and spatial examination, classic cDC1, cDC2, pDC subsets have been further refined and a novel subset of DC co-expressing IRF4 and IRF8 identified. Based on unique tissue locations within the tonsil, and close interactions with T cells (cDC1) or B cells (cDC2), DC subsets can be further subdivided by correlative phenotypic changes associated with these interactions. In addition, monocytes and macrophages expressing HLA-DR or S100AB are identified and localized in the tissue. This study thus provides a whole tissue in situ catalog of human DC subsets and their cellular interactions within spatially defined niches.

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Bolognesi, M. M., Bosisio, F. M., Manzoni, M., Schapiro, D., Tagliabue, R., Faretta, M., Parravicini, C., Haberman, A. M., Cattoretti, G.. 2018-07-16. The landscape of S100B+ and HLA-DR+ dendritic cell subsets in tonsils at the single cell level via high-parameter mapping. https://doi.org/10.1101/369983

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