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bioRxiv · 10.1101/362095

Predicting Functional Associations using Flanking Genes (FlaGs)

Saha, C. K.·Sanchez Pires, R.·Brolin, H.·Atkinson, G. C.
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Abstract

Analysis of conservation of gene neighbourhoods over different evolutionary levels is important for understanding operon and gene cluster evolution, and predicting functional associations. Our tool FlaGs (Flanking Genes) takes a list of NCBI protein accessions as in input, clusters neighbourhood-encoded proteins into homologous groups using sensitive sequence searching, and outputs a graphical visualization of the gene neighbourhood and its conservation, along with a phylogenetic tree annotated with flanking gene conservation. FlaGs has demonstrated utility for molecular evolutionary analysis, having uncovered a new toxin-antitoxin system in prokaryotes and bacteriophages. FlaGs can be downloaded from https://github.com/GCA-VH-lab/FlaGs or run at www.webflags.se.

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Saha, C. K., Sanchez Pires, R., Brolin, H., Atkinson, G. C.. 2018-07-04. Predicting Functional Associations using Flanking Genes (FlaGs). https://doi.org/10.1101/362095

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