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bioRxiv · 10.1101/356220

Increased number and activity of a lateral subpopulation of hypothalamic orexin/hypocretin neurons underlies the expression of an addicted state in rats

James, M.·Stopper, C. M.·Zimmer, B. A.·Koll, N. E.·Bowrey, H. E.·Aston-Jones, G.
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Abstract

BackgroundThe orexin system is important for reward-driven motivation but has not been implicated in the expression of a multi-phenotype addicted state.\n\nMethodsRats were assessed for economic demand for cocaine prior to and following 14d of short- (ShA), long- (LgA) or intermittent-access (IntA) to cocaine. Rats were also assessed for a number of other DSM- V-relevant addiction criteria following differential access conditions. Orexin system function was assessed by i) quantification of numbers and activity of orexin cells, ii) pharmacological blockade of the orexin-1 receptor, and iii) subregion-specific knockdown of orexin cell populations.\n\nResultsIntA produced a cluster of addiction-like behaviors that closely recapitulate key diagnostic criteria for addiction to a greater extent than LgA or ShA. IntA was associated with plasticity in orexin cell function, including increased number and activity of orexin-expressing neurons within the lateral hypothalamic (LH) subregion. This plasticity persisted during protracted withdrawal from cocaine for at least 6 months and was associated with enhanced incubation of craving. Selective knockdown of LH orexin neurons reversed the addicted state, and orexin-1 receptor signaling played a larger role in drug seeking after IntA.\n\nConclusionsThese data provide the first evidence that LH orexin system function extends beyond general reward seeking to play a critical role in the expression of a multi-phenotype addicted-like state. Thus, the orexin/hypocretin system is a potential novel target for pharmacotherapies designed to treat cocaine addiction. In addition, these data point to the IntA model as a preferred approach to modeling addictionlike behavior in rats.

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James, M., Stopper, C. M., Zimmer, B. A., Koll, N. E., Bowrey, H. E., Aston-Jones, G.. 2018-06-27. Increased number and activity of a lateral subpopulation of hypothalamic orexin/hypocretin neurons underlies the expression of an addicted state in rats. https://doi.org/10.1101/356220

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