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bioRxiv · 10.1101/347955

Nuclear speckle fusion via long-range directional motion regulates the number and size of speckles

Kim, J.·Han, K. Y.·Khanna, N.·Ha, T.·Belmont, A. S.
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Abstract

Although the formation of RNA-protein bodies has been studied intensively, their mobility and how their number and size are regulated are still poorly understood. Here, we show significant increased mobility of nuclear speckles after transcriptional inhibition, including long-range directed motion of one speckle towards another speckle, terminated by speckle fusion, over distances up to 4 um and with velocities between 0.2-1.5 m/min. Frequently, 3 or even 4 speckles follow very similar paths, with new speckles appearing along the path followed by a preceding speckle. Speckle movements and fusion events contribute to fewer but larger speckles after transcriptional inhibition. These speckle movements are not actin-dependent, but occur within chromatin-depleted channels enriched with small granules containing the speckle-marker protein SON. Our observations suggest a mechanism for long-range, directed nuclear speckle movements, contributing to overall regulation of nuclear speckle number and size as well as overall nuclear organization.

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Kim, J., Han, K. Y., Khanna, N., Ha, T., Belmont, A. S.. 2018-06-15. Nuclear speckle fusion via long-range directional motion regulates the number and size of speckles. https://doi.org/10.1101/347955

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