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bioRxiv · 10.1101/347930

Synthesis of three major auxins from glucose in Engineered Escherichia coli

Daoyi, G.·Lihua, Z.·Sijia, K.·Zhijie, L.·Xu, C.·Xun, L.·Hong, P.
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Abstract

Indole-3-acetic acid (IAA) is considered the most common and important naturally occurring auxin in plants and a major regulator of plant growth and development. In addition, phenylacetic acid (PAA) and 4-hydroxyphenylacetic acid (4HPA) can also play a role as auxin in some plants. In recent years, several microbes have been metabolically engineered to produce IAA from L-tryptophan. In this study, we showed that aminotransferase aro8 and decarboxylase kdc from Saccharomyces cerevisiae, and aldehyde dehydrogenase aldH from Escherichia coli have broad substrate ranges and can catalyze the conversion of three kinds of aromatic amino acids (L-tryptophan, L-tyrosine or L-phenylalanine) to the corresponding IAA, 4HPA and PAA. Subsequently, three de novo biosynthetic pathways for the production of IAA, PAA and 4HPA from glucose were constructed in E. coli through strengthening the shikimate pathway. This study described here shows the way for the development of agricultural microorganism for biosynthesis of plant auxin and promoting plant growth in the future.

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Daoyi, G., Lihua, Z., Sijia, K., Zhijie, L., Xu, C., Xun, L., Hong, P.. 2018-06-15. Synthesis of three major auxins from glucose in Engineered Escherichia coli. https://doi.org/10.1101/347930

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