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bioRxiv · 10.1101/347823

Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo

Ramos-Ibeas, P.·Sang, F.·Zhu, Q.·Tang, W. W. C.·Withey, S.·Klisch, D.·Loose, M.·Surani, A.·Alberio, R.
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Abstract

High-resolution molecular programs delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and then of epiblast and hypoblast in late blastocysts. We detected distinct pluripotent states, first as a short naive state followed by a protracted primed state. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.

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Ramos-Ibeas, P., Sang, F., Zhu, Q., Tang, W. W. C., Withey, S., Klisch, D., Loose, M., Surani, A., Alberio, R.. 2018-06-20. Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo. https://doi.org/10.1101/347823

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