Multiplexed Primer Extension Sequencing Enables High Precision Detection of Rare Splice Isoforms

Xu, H.; Fair, B. J.; Dwyer, Z.; Gildea, M.; Pleiss, J. A.

doi:10.1101/331629

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bioRxiv · 10.1101/331629

Multiplexed Primer Extension Sequencing Enables High Precision Detection of Rare Splice Isoforms

Xu, H.·Fair, B. J.·Dwyer, Z.·Gildea, M.·Pleiss, J. A.

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Abstract

Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for targeted RNA-sequencing that uses complex pools of reverse transcription primers to enable sequencing enrichment at user-selected locations across the genome. We demonstrate this approach by targeting hundreds to thousands of pre-mRNA splice junctions, revealing high-precision detection of splice isoforms, including rare pre-mRNA splicing intermediates.

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Xu, H., Fair, B. J., Dwyer, Z., Gildea, M., Pleiss, J. A.. 2018-05-25. Multiplexed Primer Extension Sequencing Enables High Precision Detection of Rare Splice Isoforms. https://doi.org/10.1101/331629

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