Simultaneous in vivo time-lapse stiffness mapping and fluorescence imaging of developing tissue

Thompson, A. J.; Pillai, E. K.; Dimov, I. B.; Holt, C. E.; Franze, K.

doi:10.1101/323501

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bioRxiv · 10.1101/323501

Simultaneous in vivo time-lapse stiffness mapping and fluorescence imaging of developing tissue

Thompson, A. J.·Pillai, E. K.·Dimov, I. B.·Holt, C. E.·Franze, K.

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Abstract

Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that in the developing Xenopus brain, a stiffness gradient evolves over time because of differential cell proliferation. Subsequently, axons turn to follow this gradient, underpinning the importance of time-resolved mechanics measurements.

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Thompson, A. J., Pillai, E. K., Dimov, I. B., Holt, C. E., Franze, K.. 2018-05-17. Simultaneous in vivo time-lapse stiffness mapping and fluorescence imaging of developing tissue. https://doi.org/10.1101/323501

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