Topological Skeletonization and Tree-Summarization of Neurons Using Discrete Morse Theory

Abstract

Neuroscientific data analysis has classically involved methods for statistical signal and image processing, drawing on linear algebra and stochastic process theory. However, digitized neuroanatomical data sets containing labelled neurons, either individually or in groups labelled by tracer injections, do not fully fit into this classical framework. The tree-like shapes of neurons cannot mathematically be adequately described as points in a vector space (eg, the subtraction of two neuronal shapes is not a meaningful operation). There is therefore a need for new approaches. Methods from computational topology and geometry are naturally suited to the analysis of neuronal shapes. Here we introduce methods from Discrete Morse Theory to extract tree-skeletons of individual neurons from volumetric brain image data, or to summarize collections of neurons labelled by localized anterograde tracer injections. Since individual neurons are topologically trees, it is sensible to summarize the collection of neurons labelled by a localized anterograde tracer injection using a consensus tree-shape. This consensus tree provides a richer information summary than the regional or voxel-based \"connectivity matrix\" approach that has previously been used in the literature.\n\nThe algorithmic procedure includes an initial pre-processing step to extract a density field from the raw volumetric image data, followed by initial skeleton extraction from the density field using a discrete version of a 1-(un)stable manifold of the density field. Heuristically, if the density field is regarded as a mountainous landscape, then the 1-(un)stable manifold follows the \"mountain ridges\" connecting the maxima of the density field. We then simplify this skeletongraph into a tree using a shortest-path approach and methods derived from persistent homology. The advantage of this approach is that it uses global information about the density field and is therefore robust to local fluctuations and non-uniformly distributed input signals. To be able to handle large data sets, we use a divide-and-conquer approach. The resulting software DiMorSC is available on Github[40]. To the best of our knowledge this is currently the only publicly available code for the extraction of the 1-unstable manifold from an arbitrary simplicial complex using the Discrete Morse approach.