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bioRxiv · 10.1101/321018

Evaluation of Two Sigma Transwab(R) Systems for Maintenance of Viability of Pathogenic Candida spp. Using the Clinical and Laboratory Standards Institute M40-2 A2 Standard

Elcocks, E.·Adukwu, E. C.
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Abstract

A key aspect to routine microbiology processes include the retrieval, transport and maintenance of specimens. Swab transport systems (STS) can be utilised for their low cost, ease of use and their ability to recover and maintain specimens over long durations. An increase in healthcare complications due to fungal infections raises the requirement for STS to efficiently recover and preserve pathogens of yeast origin. The Clinical and Laboratory Standards Institute (CLSI) M40-A2 protocol is used to assess the compliance of STS to a quality control standard but at present does not include the recovery of yeast. The aim of this study was to compare the results of two commercial STS and their ability to recover and maintain viability of five clinical and reference strains of Candida spp., including C. auris, when stored at room temperature and 4{degrees}C, over 48 h, using the qualitative roll plate method. Findings from this study indicate that the STS used in this study are suitable for the collection and maintenance of the Candida spp. tested, and is very suitable for the recovery of clinical C. auris.

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Elcocks, E., Adukwu, E. C.. 2018-05-13. Evaluation of Two Sigma Transwab(R) Systems for Maintenance of Viability of Pathogenic Candida spp. Using the Clinical and Laboratory Standards Institute M40-2 A2 Standard. https://doi.org/10.1101/321018

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