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bioRxiv · 10.1101/317917

Changing Times: Fluorescence-lifetime Analysis of Amyloidogenic SF-IAPP Fusion Protein

Antimonova, O. I.·Lebedev, D. V.·Zabrodskaya, Y. A.·Grudinina, N. A.·Shavlovsky, M. M.·Egorov, V. V.
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Abstract

The fluorescence lifetime of the superfolder green fluorescent protein (SF) and the SF protein fused with islet amyloid polypeptide (SF-IAPP) were studied in polyacrylamide gel. It was shown that the SF average fluorescence lifetime under these conditions slightly differs from that of the SF-IAPP monomer. SF-IAPP does not lose the ability to form amyloid-like fibrils; meanwhile, the average fluorescence lifetime of the fusion protein in fibrils is reduced. We propose the application of Fluorescent-lifetime Imaging Microscopy (FLIM) to the measurement of average fluorescence lifetimes of fusion proteins (amyloidogenic protein-SF) in the context of studies using cellular models of conformational diseases.

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Antimonova, O. I., Lebedev, D. V., Zabrodskaya, Y. A., Grudinina, N. A., Shavlovsky, M. M., Egorov, V. V.. 2018-05-09. Changing Times: Fluorescence-lifetime Analysis of Amyloidogenic SF-IAPP Fusion Protein. https://doi.org/10.1101/317917

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